论文部分内容阅读
采用新型载体（ＬａｍｂｄａＧＥＭＲ－１１ｖｅｃｔｏｒ系统）完成了杜氏利什曼原虫四川人分离株（简称Ｌ．ｄ．四川人株）的基因组ＤＮＡ大片段克隆。重组噬菌体总数为１．５×１０６。采用地高辛标记的Ｌ．ｄ．四川人株ＳＳＵｒＤＮＡ扩增产物探针筛选文库，获得３个含有Ｌ．ｄ．四川人株ｒＤＮＡ插入片段的重组噬菌体克隆，为今后的亚克隆和ＳＳＵｒＤＮＡ可变区及间隔区结构和功能研究打下了基础。
The large genomic DNA clone of Leishmania donovani Sichuan isolate (L.d. Sichuan strain) was completed with a novel vector (LambdaGEMR-11vector system). The total number of recombinant phage was 1.5 × 106. Digoxigenin-labeled L. d. Sichuan strain SSUrDNA amplification product probe screening library, obtained three containing L. d. The recombinant phage clones of the rDNA insert of Sichuan strain laid the foundation for future subcloning and SSU rDNA variable region and spacer structure and function studies.