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目的:观察白藜芦醇对大鼠局灶性脑缺血再灌注损伤的治疗作用及可能的机制。方法:将SD大鼠随机分为2组:对照组(n=16),白藜芦醇组(n=16)。对照组再灌注即刻腹腔给予0.5 ml生理盐水,白藜芦醇组再灌注即刻腹腔给予20 mg/kg白藜芦醇。再灌注22小时后,进行神经功能学评分、脑梗死容积测定,用分光光度仪测定脑组织溶浆中SOD、MDA和MPO的含量。结果:再灌注22小时后,白藜芦醇治疗组可以改善大鼠神经功能学评分和降低脑梗死面积(P<0.05),同时可以增加脑组织溶浆中SOD的活性,降低MDA和MPO的含量。结论:白藜芦醇通过减轻白细胞的浸润、提高自由基的清除率对大鼠局灶性脑缺血再灌注损伤发挥治疗作用。
Objective: To observe the therapeutic effect and possible mechanism of resveratrol on focal cerebral ischemia-reperfusion injury in rats. Methods: SD rats were randomly divided into two groups: control group (n = 16), resveratrol group (n = 16). The control group was intraperitoneally administered with 0.5 ml saline immediately after reperfusion, resveratrol 20 mg / kg resveratrol was given intraperitoneally immediately after reperfusion. 22 hours after reperfusion, neurological score and cerebral infarction volume were measured. The contents of SOD, MDA and MPO in cerebral tissue were measured by spectrophotometer. Results: After resuscitation for 22 hours, the resveratrol treatment group could improve neurological score and reduce the infarct size (P <0.05), and at the same time, increase the activity of SOD and decrease the contents of MDA and MPO content. Conclusion: Resveratrol plays a therapeutic role in focal cerebral ischemia-reperfusion injury in rats by alleviating the infiltration of leucocytes and increasing the clearance rate of free radicals.