我们探索建立一种 HCV基因分型方法 ,用 5’非编码区 (5’NCR) 1,2 ,3,1b型特异性引物 ,应用逆转录套式 PCR,对 HCV RNA阳性血清进行分型。对 6 5份 HCV RNA阳性血清的分型率为 90 .8% (5 9/6 5 )。 5 9例中混合感染 7例 ,1型 5 0例 ,其中 1b亚型 46例 ;3型 2例。从而认为 :所用的型特异性引物可成功地对 HCV基因分型 ,分型结果证实南京市 HCV流行优势株为 1b亚型。 We explored the establishment of an HCV genotyping method using reverse transcriptase-mediated polymerase chain reaction (PCR) of 5 ’non-coding region (5’NCR) of type 1, 2, 3, and 1b specific primers to classify HCV RNA positive sera. The typing rate of 65 HCV RNA positive sera was 90.8% (59/65). Among 59 cases, 7 cases were mixed infection, 50 cases were type 1, of which 46 cases were type 1b and 2 cases were type 3. Therefore, it is concluded that the genotype-specific primers used in this study can successfully genotype HCV, and the genotyping results confirmed that the HCV predominant strain in Nanjing is 1b subtype.