本文使用黄嘌呤-黄嘌呤氧化酶—鲁米诺化学发光体系和化学发光检测法研究了黄芪中各成分的清除超氧阴离子自由基的能力,以药物抑制发光强度50%的浓度(IC_(50))为指标。经研究证明,黄芪总皂甙部分(N)具有较强的活性,IC_(50)为185μg/ml,再经进一步导向分离并鉴定证明,黄芪甙Ⅲ,Ⅳ和Ⅵ的 IC)_(50)分别为80、50和11μg/ml,从而证明黄芪的抗心力衰竭的有效成分可能为黄芪总皂甙部分。 In this study, xanthine-xanthine oxidase-luminol chemiluminescence system and chemiluminescence detection method were used to study the ability of various components of Scutellaria baicalensis to scavenge superoxide anion free radicals, and the concentration of drug to inhibit luminescence intensity was 50% (IC_(50) )) as an indicator. Studies have shown that the total saponin fraction of Astragalus (N) has strong activity, IC50 is 185 μg/ml, and after further guidance separation and identification, the IC of the Astragalus III, IV, and VI) (50) are respectively For 80, 50 and 11 μg/ml, it was proved that the effective component of anti-heart failure of Astragalus may be the total saponin part of Astragalus membranaceus.