氨苄青霉素及钠盐的含量测定方法有硝酸汞-电位滴定法、生物法、剩余碘量法。最新版的美国药典改用高效液相色谱法(HPLC)。现多采用咪唑汞-分光光度法,但该法稀释步骤多,又需准确控制加试剂的量、pH值,水浴温度和时间,精密度差,费时费物。本文采用反相HPLC法,以咖啡因为内标,用峰面积比值对氨苄青霉素进行定量测定,结果令人满意。 Ampicillin and sodium salt content determination of mercury nitrate - potentiometric titration, biological method, residual iodine method. The latest edition of the United States Pharmacopoeia switched to high performance liquid chromatography (HPLC). Is the use of imidazole mercury - spectrophotometry, but the method of dilution and more, but also need to accurately control the amount of reagent, pH, water bath temperature and time, poor precision, time-consuming materials. In this paper, reversed-phase HPLC method to caffeine as an internal standard, with a peak area ratio of ampicillin quantitative determination, the results are satisfactory.