淋巴结组织结构复杂,细胞成分多,HE染色干扰因素多,不易制出理想的组织切片。往往切片偏厚、易碎和染色灰、胞核不清晰是常出现的问题,严重影响其常规制片的质量,给病理诊断带来困难,造成误诊~([1])。淋巴结的制片方法一直是病理技术的难题~([2]),制作出优良的切片对诊断淋巴结疾病至关重要。本文就淋巴结HE染色的干扰因素及其 Lymph node tissue structure is complex, many cellular components, HE staining interference factors, not easy to make the ideal tissue sections. Often slices thick, fragile and staining gray, unclear nucleus is a common problem that seriously affect the quality of its conventional production, to the pathological diagnosis difficulties, resulting in misdiagnosis (1). Lymphangiogenesis has been a difficult problem in pathology ~ ([2]) and producing excellent sections is crucial for the diagnosis of lymph node disease. In this paper, HE staining of lymph nodes and its interference factors