复方制剂中毒时,制剂中各成份间的干扰常给法医化学分析带来一定的困难。而常用的排除干扰方法既费时又要消耗大量较为昂贵的有机溶剂。本文探讨了在一阶导数光谱上应用双波长法,测定脑清片中毒时血中的咖啡因含量,方法简易、准确、在282mm和254mm两波长处,咖啡因的一阶导数之差与其波度有理想的线性关系,(?)=0.9999。咖啡因在血中的平 Compound preparation poisoning, the preparation of the interference between the ingredients often give forensic chemical analysis to bring some difficulties. Commonly used methods to eliminate interference are both time-consuming and consume a large amount of more expensive organic solvents. In this paper, the dual-wavelength method was applied to the first-order derivative spectroscopy to determine the caffeine content in the blood of poisoning brain slices. The method was simple and accurate. The difference between the first derivative of caffeine and its wave at 282mm and 254mm wavelength Degree has an ideal linear relationship, (?) = 0.9999. Caffeine in the blood of the flat