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肠球菌可以引起人的严重疾病,并且对化学治疗剂有一定的抗药性。因此,需要快速鉴别这些微生物与其他链球菌。为此,作者研究了一种可以快速鉴定肠球菌的氯化钠-七叶苷水解试验。本试验培养液系在1000ml蒸馏水中含有2.0g七叶苷,0.5g枸橼酸铁铵,50.0g氯化钠,0.4g K_2HPO_4和0.1g KH_2PO_4。pH值调至5.6±0.2。将0.5ml溶液装入试管(12×75mm)中并保存于4℃。在这些条件下,原液至少可以稳定保存8周。按接近McFarland标准3的密度将所有培养物接种到0.5ml缓冲七叶苷溶液中并于35℃孵育4小时。溶液的颜色变成深褐色或黑色表明试
Enterococcus can cause serious human diseases, and have some resistance to chemotherapeutic agents. Therefore, there is a need to rapidly identify these microbes with other streptococci. To this end, the authors studied a sodium chloride-aesculetin hydrolysis test for rapid identification of enterococci. The test medium contains 2.0g esculin, 0.5g ferric ammonium citrate, 50.0g sodium chloride, 0.4g K 2 HPO 4 and 0.1g KH 2 PO 4 in 1000ml distilled water. The pH is adjusted to 5.6 ± 0.2. 0.5 ml of the solution was placed in a test tube (12 x 75 mm) and stored at 4 ° C. Under these conditions, the stock solution is stable for at least 8 weeks. All cultures were inoculated into 0.5 ml bucines solution at a density approaching McFarland standard 3 and incubated for 4 hours at 35 ° C. The color of the solution turns dark brown or black indicates that the test