论文部分内容阅读
目的探讨碱性成纤维细胞生长因子（ｂＦＧＦ）的异常表达与胶质瘤的恶性程度和增殖活性的关系。方法采用Ｎｏｒｔｈｅｒｎ杂交和免疫组化法对５７例人脑胶质瘤进行ｂＦＧＦ基因表达及Ｋｉ－６７标记指数检测及相关性分析。结果各组胶质瘤均有ｂＦＧＦｍＲＮＡ表达，且表达水平以恶性胶质瘤为高。Ｋｉ－６７标记指数在胶质瘤中升高显著。ｂＦＧＦｍＲＮＡ表达水平与胶质瘤恶性度及Ｋｉ－６７标记指数呈正相关。结论ｂＦＧＦ与胶质瘤的恶性进展有关，可作为判定胶质瘤恶性度的指标之一。
Objective To investigate the relationship between the abnormal expression of basic fibroblast growth factor (bFGF) and the malignant degree and proliferative activity of glioma. Methods Northern blot and immunohistochemistry were used to detect bFGF gene expression and Ki-67 labeling index in 57 human gliomas and their correlation analysis. Results bFGF mRNA expression was observed in all groups of gliomas, and the expression level was high in malignant gliomas. The Ki-67 marker index was significantly elevated in gliomas. The bFGF mRNA expression level was positively correlated with the glioma malignancy and Ki-67 labeling index. Conclusion bFGF is related to the malignant progression of gliomas and it can be used as one of the indicators to determine the malignancy of gliomas.