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目的:组织工程骨移植的排斥反应将主要取决于成骨细胞的抗原成分,对不同种系成骨细胞植入后体液免疫水平进行检测,探讨组织工程骨组织植入体内后可能的免疫排斥机制,并为应用于工程骨的种子细胞寻找可靠的种系来源。方法:行成骨细胞同胎、间种异体、同种异基因及自体细胞腹直肌袋内移植,分别于术后1,2,4,8周行免疫学及组织形态学检测。结果:各组动物在移植后一两周内均可检测到特异性抗体,同种异基因移植组持续时间最久,异基因组组织学指标高于同种异体组。结论:自体、同胎及同种异体或同种异基因细胞均可作为组织工程骨移植可靠的细胞来源。
OBJECTIVE: The rejection of tissue engineered bone graft will mainly depend on the antigenic components of osteoblasts. The level of humoral immunity after osteoblast implantation in different germ cells is tested to explore the possible mechanism of immune rejection after implantation of tissue engineered bone tissue in vivo , And find reliable germ line sources for seed cells used in engineered bones. Methods: Allograft, allogeneic, allogeneic and autologous cells were transplanted into the rectus abdominis muscle of osteoblasts. Immunohistochemistry and histomorphology were performed at 1, 2, 4 and 8 weeks after operation. Results: The specific antibodies could be detected within one to two weeks after transplantation. The allogeneic transplantation group had the longest duration and the allogeneic histological indexes were higher than allogeneic groups. CONCLUSION: Autologous, identical fetus and allogeneic or allogeneic cells can be used as a reliable source of cells for tissue engineered bone graft.