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以宜良宝洪茶、莽水大叶原头茶、波上金苔、云抗 10号 4个云南茶树品种为材料 ,对其DNA提取和RAPD分子标记方法进行了研究。结果表明 :所采用的经改进的CTABDNA微量提取法 ,可以得到高质量的茶树DNA ,分子量大于 2 1kb ,可满足RAPD扩增 ;用 18个不同的随机引物对所提取的 4个品种基因组DNA进行了RAPD分子标记分析 ,其中 3个 (占 16 6 7% )引物在茶树品种间可扩增出多态性产物。建立了云南茶树DNA微量、快速提取和RAPD标记的分析程序 ,为RAPD分析应用于云南茶树遗传研究打下了良好的基础
The methods of DNA extraction and RAPD molecular marker were studied in four Yunnan tea cultivars, including Yiliangbao Hongcha, Mangshui Da Yewuan Toucha, Porphyrata arborensis and Yunkang 10. The results showed that the modified CTAB DNA micro-extraction method could be used to obtain high-quality DNA of Camellia, with a molecular weight greater than 21kb, which could be used for RAPD amplification. Genomic DNA of the four cultivars extracted with 18 different random primers RAPD analysis of the molecular markers, of which three (16 6 7%) primers in the tea varieties can be amplified polymorphic products. The establishment of Yunnan tea tree DNA trace, rapid extraction and RAPD markers analysis program for the RAPD analysis of Yunnan tea tree genetic research laid a good foundation