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目的：本研究旨在探讨Resveratrol对人T 淋巴细胞活化的影响及其分子机制。方法在体外使用Resveratrol处理人外周血T 细胞，并给予抗人CD3和抗CD28抗体活化，观察其对T细胞增殖、细胞因子分泌及细胞凋亡的影响。同时进一步通过免疫印迹和免疫共沉淀的方法探讨Resveratrol对T细胞活化影响的分子机制。结果我们现有的实验提示Resveratrol可以明显抑制人外周血T 淋巴细胞的增殖，且能抑制IL-2、IL-4、IL-5、IFNγ等细胞因子的分泌；但并不影响T细胞的凋亡。进一步研究表明Resveratrol通过提高Sirt1的表达和增强其对C-jun的脱乙酰化来发挥作用。结论此研究提示resveratrol可能在T细胞引起的炎症如自身免疫性疾病治疗中发挥重要作用。“,”Object: We aim to explore the function and molecular mechanisms of resveratrol on human T cellactivation. Method: The PBMC were obtained from health people, and purified by Ficol-hypaque, the T cellwere activated by anti-CD3 and anti-CD28 antibodies, the proliferation was evaluated by CFSE celldivision assay and Brdu cellproliferation assay. The levels of cytokines in activated T cells culture supernatant were measured by ELISA. The expression of Sirt1 and alteration of acetylation of C-Jun were evaluated by western-blot. Result:We found that Resveratrol significantly inhibited the activation and cytokines production of T cellin hunman PBMC. The expression of Sirt1 was up-regulated and acetylation of C-Jun was decreased in resveratrol-treated T cells. Conclusion: Resveratrol inhibited the T cellactivation by up-regulating the expression of Sirt1 and enhancing the deacetylase activity of Sirt1 on C-Jun in T cells.