论文部分内容阅读
目的观察肝细胞癌组织中miR-141的表达水平,通过上调MHCC-97H肝癌细胞中miR-141的水平观察对MHCC-97H细胞增殖、侵袭及迁移的影响。方法实时定量PCR检测miR-141在肝癌及癌旁组织中的表达水平,并分析miR-141表达水平与临床病理指标的关系及生存率。利用人工合成的miR-141模拟物,瞬时转染MHCC-97H细胞。MTT法检测MHCC-97H细胞的增殖,TranswellTM实验检测MHCC-97H细胞的侵袭及迁移,Westernblot法及免疫组织化学检测miR-141下游潜在靶点肝细胞产生的促红素受体A2(EphA2)的表达。结果miR-141在肝癌组织中表达水平显著低于相应癌旁组织;肝癌组织中miR-141低表达与TNM分期、门静脉侵犯及Edmondson分级显著相关;低表达miR-141患者3年生存率明显低于高表达组;miR-141模拟物转染可上调MHCC-97H细胞miR-141水平,上调miR-141可显著抑制MHCC-97H细胞的增殖、侵袭及迁移,并下调EphA2的蛋白水平;miR-141低表达组EphA2蛋白水平明显高于miR-141高表达组,相关性分析显示肝癌组织中miR-141与EphA2蛋白表达呈显著负相关。结论miR-141在肝癌组织中表达下调且与EphA2表达呈显著负相关,其低表达与肝癌恶性临床病理特征有关,上调miR-141表达可抑制EphA2的表达抑制肝癌细胞增殖、侵袭及迁移能力。
Objective To observe the expression of miR-141 in hepatocellular carcinoma (HCC) and to observe the effect of miR-141 on the proliferation, invasion and migration of MHCC-97H cells by up-regulating the level of miR-141 in MHCC-97H hepatoma cells. Methods Real-time quantitative PCR was used to detect the expression of miR-141 in hepatocellular carcinoma and its adjacent tissues. The relationship between miR-141 expression and clinicopathological parameters and survival rate were analyzed. MHCC-97H cells were transiently transfected with the synthetic miR-141 mimics. The proliferation of MHCC-97H cells was detected by MTT assay. The invasion and migration of MHCC-97H cells were detected by TranswellTM assay. The expression of EphA2 in potential hepatocytes downstream of miR-141 was detected by Western blot and immunohistochemistry expression. Results The expression of miR-141 in HCC tissue was significantly lower than that in corresponding paracancerous tissues. The low expression of miR-141 in HCC tissues was significantly associated with TNM staging, portal vein invasion and Edmondson grade. The 3-year survival rate of miR-141 patients with low expression was significantly lower MiR-141 mimics could up-regulate the expression of miR-141 in MHCC-97H cells. Up-regulation of miR-141 could significantly inhibit the proliferation, invasion and migration of MHCC-97H cells and down-regulate the expression of EphA2 protein. MiR- 141 low expression of EphA2 protein levels were significantly higher than miR-141 high expression group, correlation analysis showed that the expression of miR-141 and EphA2 protein in liver cancer was significantly negatively correlated. Conclusions The expression of miR-141 is down-regulated in HCC and negatively correlated with the expression of EphA2. The low expression of miR-141 is associated with the clinicopathological features of HCC. Up-regulating the expression of miR-141 can inhibit the expression of EphA2 and inhibit the proliferation, invasion and migration of HCC.