将紫外线照射后的宁南2 号根瘤菌(Rhizobium legum inosarum Linglan 2)在不同光照和温度培养下表明,光照强度越大,照射时间越长,根瘤菌损伤就越大,存活率就越低,生长速度就越慢,而且光照还会降低突变株的耐酸碱特性。虽然培养温度对根瘤菌存活率和耐酸耐碱特性影响不大,但根瘤菌的生长和分裂速度则与温度有关。由此说明,培养条件在根瘤菌突变体的筛选中具有重要作用 Rhizobium legum inosarum Linglan 2 irradiated by ultraviolet light under different light and temperature showed that the higher the light intensity and the longer the irradiation duration, the greater the rhizobia damage and the lower the survival rate. The slower the growth rate, and light will reduce the mutant acid-base properties. Although culture temperature had little effect on the survival rate of Rhizobia and the properties of acid and alkali resistance, the growth and division rate of rhizobia were related to temperature. Thus, culture conditions play an important role in the screening of rhizobia mutants