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通过人工液体浅层培养方式培养猪苓菌丝体,用热水浸提法提取猪苓菌丝体多糖(PPS1),经纯化测定其糖含量,用红外光谱分析鉴定多糖;纯化鉴定的PPS1,通过小鼠进行腹腔单核巨噬细胞功能测定试验、E玫瑰花环试验、足跖肿胀厚度试验、淋巴细胞转化试验、EAC花环试验等5项免疫学试验初步探讨其免疫药理学作用,同时与猪苓菌核多糖(PPS2)进行比较。试验数据经统计学分析显示,E玫瑰花环试验中PPS1与空白对照组相比差异显著(P<0.05);腹腔单核巨噬细胞功能测定试验、足跖肿胀厚度试验、淋巴细胞转化试验及EAC花环试验PPS1与空白对照组相比差异极显著(P<0.01);5项免疫学试验PPS1与PPS2相比差异均不显著。结果表明,PPS1能明显提高小鼠的免疫功能。
The mycelium of Polyporus umbellatus was cultured by artificial liquid shallow culture, the polysaccharide of Polyporus umbellatus (PPS1) was extracted by hot water extraction, the content of polysaccharide was determined by purification, and the polysaccharide was identified by infrared spectroscopy. Purified and identified PPS1, Five immunological tests including intraperitoneal monocyte-macrophage function test, E-rosette test, plantar swelling test, lymphocyte transformation test and EAC garland test were conducted to investigate the immunopharmacological effects in mice. Ling Ling nuclear polysaccharide (PPS2) were compared. The statistical analysis of the experimental data showed that there was a significant difference (P <0.05) between PPS1 and control group in the E-rosette test; the function test of peritoneal monocyte-macrophage, the plantar swelling test, lymphocyte transformation test and EAC There was significant difference between rosette test PPS1 and blank control group (P <0.01). There was no significant difference between PPS1 and PPS2 in five immunological tests. The results showed that PPS1 can significantly improve immune function in mice.