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采用观察MDCK细胞病理变化 (CPE)和测定培养液中红细胞吸附效价 (HA)的方法对黄芪赤风口服液 (简称黄赤液 )对甲、乙两型流感病毒株的抑制作用进行了研究。结果表明 :用黄赤液先处理细胞后攻击病毒 ,或病毒先感染细胞后加黄赤液或黄赤液与病毒同时加入 ,1∶80稀释的黄赤液均能明显保护MDCK细胞 ,抑制甲型流感病毒A/PR/ 8/ 34及乙型流感病毒B/沪防 / 14 / 95的复制 ,降低流感病毒感染滴度 1.5～ 2个log以上。提示黄芪赤风口服液在体外有明显的抗流感病毒的作用。
The inhibitory effect of Huangqi Chifeng Oral Liquid (Huang Red Solution) on influenza A and B virus strains was investigated by observing MDCK cell pathological changes (CPE) and measuring the red cell adsorption titer (HA) in the culture fluid. . The results showed that the virus was infected after the first treatment of the cells with Huanghongye, or the virus was infected first and then the yellow red liquor or Huang red liquor was added simultaneously with the virus. The 1:80 diluted Huanghongye could obviously protect the MDCK cells. Replication of Influenza A/PR/8/34 and Influenza B/Shanghai/14/95 reduces the influenza virus infection titer by 1.5 to 2 log or more. It is suggested that Huangqi Chifeng Oral Liquid has obvious anti-influenza virus effect in vitro.