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应用放射免疫分析（ＲＩＡ）技术测定了实验性大鼠脊髓创伤后各段脊髓组织中３种不同内源性阿片肽含量的动态变化。结果表明：强啡肽（ＤｙｎＡ）随损伤程度的加重进行性升高，ＤｙｎＡ含量变化局限于损伤胸段及邻近腰段脊髓，且呈时间依赖型变化。β－内啡肽（β－Ｅｐ）无明显变化。亮脑啡肽（Ｌ－Ｅｋ）伤后在损伤段脊髓组织中含量降低或无明显变化，且与受伤程度无明显相关。研究结果证明：内源性阿片肽参与了实验性大鼠脊髓创伤后双后肢体运动功能丧失的病理过程，且以其中ＤｙｎＡ的作用最重要。
Radioimmunoassay (RIA) technique was used to determine the dynamic changes of three endogenous opioid peptides in spinal cord after spinal cord injury in experimental rats. The results showed that dynorphin (DynA) increased progressively with the increase of injury degree. The change of DynA content was limited to the injured thoracic and adjacent lumbar spinal cord, and it showed a time-dependent change. β-endorphin (β-Ep) no significant change. Brilliant enkephalin (L-Ek) injury in spinal cord injury in the spinal cord tissue content decreased or no significant change, and no significant correlation with the degree of injury. The results demonstrate that endogenous opioid peptide is involved in the pathological process of motor function loss of bilateral hindlimbs after experimental spinal cord injury in rats, and DynA plays the most important role.