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目的建立高效液相色谱法同时测定人参健脾丸中人参皂苷Rg_1、Re、Rb_1和酸枣仁皂苷A、B 5种有效成分的含量。方法采用Eclipse XDB C_(18)(4.6 mm×250 mm,5μm)色谱柱,柱温35℃,以乙腈-水为流动相梯度洗脱,分段变体积流量测定,检测波长203 nm。结果人参皂苷Rg1、Re、Rb1和酸枣仁皂苷A、B分别在0.32~2.24μg(r=0.998 2)、0.16~1.12μg(r=0.995 3)、0.32~2.24μg(r=0.999 6)、0.16~1.12μg(r=0.991 5)、0.08~0.56μg(r=0.999 6)内与色谱峰面积呈良好的线性关系。平均回收率分别为97.18%、97.62%、98.79%、98.48%和94.51%,RSD分别为1.10%、0.98%、0.34%、1.09%和1.88%。结论首次建立的梯度洗脱同时检测人参皂苷Rg1、Re、Rb1和酸枣仁皂苷A、B含量方法准确、可靠,重复性好,可用于人参健脾丸的质量控制。
OBJECTIVE To establish a HPLC method for the simultaneous determination of 5 active ingredients of ginsenosides Rg_1, Re, Rb_1 and jujuboside A, B in ginseng and spleen pills. Methods Eclipse XDB C_ (18) (4.6 mm × 250 mm, 5 μm) column was used. The column temperature was 35 ℃. The gradient elution was carried out with acetonitrile-water as mobile phase. Results Ginsenosides Rg1, Re, Rb1 and Jujuboside A, B were 0.32-2.24μg (r = 0.9982), 0.16-1.12μg (r = 0.995 3), 0.32-2.24μg 0.16 ~ 1.12μg (r = 0.991 5), 0.08 ~ 0.56μg (r = 0.999 6) and the chromatographic peak area showed a good linear relationship. The average recoveries were 97.18%, 97.62%, 98.79%, 98.48% and 94.51%, respectively, with RSDs of 1.10%, 0.98%, 0.34%, 1.09% and 1.88%, respectively. Conclusion The first established gradient elution assay of ginsenosides Rg1, Re, Rb1 and Jujuboside A, B content is accurate, reliable, reproducible, and can be used for quality control of ginseng spleen pill.