论文部分内容阅读
目的：研究微卫星不稳定性（MSI）在胃癌发生中的作用及其与bcl－2、erbB－2和突变型p53蛋白表达的关系。方法：采用PCR－SSLP和免疫组织化学技术检测了50例手术切除胃癌和15例肠化组织标本的MSI和bcl－2、erbB－2及突变型p53蛋白表达。结果：胃癌MSI发生率为54％，肠化组织MSI发生率为20％，胃癌组织bcl－2、erbB、及p53蛋白阳性率分别为60％、22％和38％，肠化生组织bcl－2蛋白表达阳性率为53．3％，而p53和erbB－2均为阴性。高中分化腺癌MSI发生率（86．7％）显著高于低分化腺癌（38．7％）（P＜0．05），肠型胃癌MSI发生率（77．8％）显著高于胃型胃癌（40％）（P＜0．05）；MSI与胃癌患者年龄、性别、肿瘤的部位、大小、淋巴结转移、浆膜浸润、临床分期及bcl－2、erbB－2和突变型p53蛋白表达无显著相关。结论：MSI在胃癌的发生中可能起重要作用，可能是胃癌发生过程中的一个早期分子改变，其致癌机制不同于bcl－2、erbB－2及p53。
Objective: To investigate the role of microsatellite instability (MSI) in the development of gastric cancer and its relationship with the expression of bcl-2, erbB-2 and mutant p53 protein. METHODS: The expression of MSI, bcl-2, erbB-2 and mutant p53 protein in 50 cases of surgically resected gastric carcinoma and 15 cases of intestinal metaplasia were detected by PCR-SSLP and immunohistochemistry. Results: The incidence of MSI in gastric cancer was 54%. The incidence of MSI in intestinal metaplasia was 20%. The positive rates of bcl-2, erbB, and p53 in gastric cancer were 60%, 22%, and 38%, respectively. The intestinal metaplasia bcl- 2 The positive rate of protein expression was 53.3%, but both p53 and erbB-2 were negative. The incidence of MSI in high-grade differentiated adenocarcinoma (86.7%) was significantly higher than that of poorly differentiated adenocarcinoma (38.7%) (P<0.05). The incidence of MSI in intestinal-type gastric cancer (77.8%) was significantly higher than that of stomach. Gastric cancer (40%) (P<0.05); Age, sex, tumor location, size, lymph node metastasis, serosal invasion, clinical stage, and bcl-2, erbB-2, and mutant p53 protein in MSI and gastric cancer patients Expression was not significantly related. CONCLUSIONS: MSI may play an important role in the development of gastric cancer. It may be an early molecular alteration during the development of gastric cancer. Its mechanism of oncogenicity is different from that of bcl-2, erbB-2 and p53.