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1990年初广州市某医科大学附属医院显微外科实验室发生3例EHF病人,经流行病学调查、病原学和血清学证实是由实验大白鼠引起的EHF暴发。从病人急性期血清及实验大白鼠肺分离获得两株EHFV,应用北京病毒所EHF病毒MCAb分型试剂检测,证实属家鼠型病毒;3例患者的恢复期血清抗体滴度有4倍或4倍以上升高;在实验大白鼠也查到EHF抗体。从病人血清及大白鼠肺分离到两株EHFV,在广东省尚属首次,为广州市EHF监测提供佐证,同时也表明EHF病毒McAb试剂对分型的鉴别诊断有实用价值。
At the beginning of 1990, three patients with EHF in microsurgery laboratory of a medical university affiliated hospital in Guangzhou city were confirmed by epidemiology, etiology and serology to be EHF outbreak caused by experimental rats. Two strains of EHFV were isolated from the lungs of patients with acute phase and experimental rat lungs. They were detected by EHF virus MCAb typing reagent of Beijing virus and confirmed to be a domestic mouse-type virus. The serum antibody titers of convalescent patients were 4 times or 4 times Times higher than that in the experimental rats were also found EHF antibody. EHFV isolates from patients’ sera and rat lungs are the first in Guangdong province to provide evidence for monitoring EHF in Guangzhou City. At the same time, it is also indicated that EHF virus McAb reagent has practical value in the differential diagnosis of typing.