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为了解河南省奶牛传染性鼻气管炎病毒(Infectious bovine rhinotracheitis virus,IBRv)的感染情况,采用SYBR Green I荧光定量PCR方法,对郑州、新乡、焦作等地区采集的397头奶牛鼻腔、阴道棉拭子进行了IBRv DNA检测。结果:总体检出率为7.1%,检出率最高场可达73.7%;患病牛群中的检测率(6.5%)高于外观健康牛群中的阳性率(5.9%)。由此证实,河南省奶牛场中存在一定的IBRV感染,并且其感染程度与年龄、机体健康状况、牛场饲养管理和卫生情况具有一定的相关性。
In order to understand the infection status of Infectious bovine rhinotracheitis virus (IBRv) in Henan Province, 397 dairy cows collected from Zhengzhou, Xinxiang and Jiaozuo were collected with nasal and vaginal swabs by SYBR Green I quantitative PCR The IBRv DNA test was performed. Results: The overall detection rate was 7.1%, and the highest detection rate was 73.7%. The detection rate in ill animals (6.5%) was higher than that in healthy cattle (5.9%). This confirmed that there is a certain degree of IBRV infection in dairy farms in Henan Province, and the degree of infection is related to age, body health status, cattle farm management and health status.