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报道可见吸收光谱线型的高斯多峰拟合计算,用于研究非离子表面活性剂吐温-80(Polysorbate 80,简称Tween80)和阴离子表面活性剂十二烷基苯磺酸钠(sodium dodecyl benzene sulfonate,SDBS)混合水溶液中的聚集行为。以孔雀石绿(malachite green,MG)为探针,测量一系列不同浓度的Tween80-SDBS-MG水溶液的可见吸收光谱,其特征是MG单体吸收峰(619～632)nm和二聚体吸收峰(590～606)nm叠合在一起。用Origin软件频谱分析软件包高斯多峰拟合法,计算出体系可见光谱吸收叠合峰的分峰、峰面积(积分吸光度)、频移及半高宽等光谱的线型参数;单体和二聚体峰面积比(相对积分吸光度A_2/A_1)、频移(Δλ)及半高宽(w_1,w_2)使Tween80或SDBS浓度图形,在临界胶束浓度cmc处发生突变。首次发现体系可见吸收光谱线型参数半高宽对吐温-80-十二烷基苯磺酸钠混合表面活性剂相互作用和聚集行为敏感,并成功用于研究该混合表面活性剂体系的聚集行为。
It is reported that Gaussian multi-peak fitting calculation of absorption spectrum can be used to study the effect of non-ionic surfactant Tween-80 (Tween 80) and anionic surfactant sodium dodecyl benzene sulfonate, SDBS) in aqueous solution. The visible absorption spectra of a series of different concentrations of aqueous Tween80-SDBS-MG solution were measured with malachite green (MG) as a probe, which was characterized by the absorption peak of MG monomer (619-632 nm) and dimer absorption Peak (590 ~ 606) nm superimposed together. The Gaussian multi-peak fitting method was used to calculate the spectral parameters of the peak, the peak area (integral absorbance), the linear parameters of frequency shift and FWHM. The monomer and bis The peak area ratio (relative integral absorbance A_2 / A_1), frequency shift (Δλ) and full width at half maximum (w_1, w_2) of Tween80 or SDBS concentration curves were mutated at the critical micelle concentration cmc. It was found for the first time that the half width of absorption linear parameter was sensitive to the interaction and aggregation behavior of Tween-80-sodium dodecyl benzene sulfonate surfactant and was successfully used to study the aggregation of the surfactant system behavior.