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小白菜品种矮脚黄生长在光照培养室内,在以0.0、13.0kJm-2d-1(模拟臭氧浓度下降20%时的UV-B强度)的紫外光B(UV-B280~320nm)进行4d与7d的照射处理,研究UV-B对小白菜叶片内的类黄酮和膜脂质过氧化作用的影响。研究结果表明,UV-B辐射诱导了小白菜叶片内吸收UV的类黄酮化合物的积累。同时UV-B辐射降低小白菜叶片内抗坏血酸的含量,13.0kJm-2d-1的UV-B辐射显著抑制了小白菜叶片内过氧化氢酶和SOD酶的活性。同时UV-B辐射导致小白菜叶片内丙二醛的积累。UV-B辐射对以上生化指标的影响随处理时间的延长而加深。上述结果表明,UV-B辐射加深了小白菜的膜脂质过氧化作用,而吸收UV的类黄酮的积累并不能完全缓解UV-B的伤害。
The Chinese cabbage cultivar Dwarf Yellow was grown in a light culture chamber. UVB B (UV-B280-320 nm) with 0.0,13.0 kJm-2d-1 (UV-B intensity at 20% reduction in simulated ozone concentration) The effects of UV-B on flavonoids and membrane lipid peroxidation in Chinese cabbage leaves were studied under irradiation of 4d and 7d. The results showed that UV-B radiation induced the absorption of flavonoids in cabbage leaves. At the same time UV-B radiation reduced the content of ascorbic acid in cabbage leaves. The UV-B radiation of 13.0kJm-2d-1 significantly inhibited the activity of catalase and SOD in cabbage leaves. At the same time UV-B radiation led to the accumulation of MDA in cabbage leaves. The effects of UV-B radiation on the above biochemical indicators deepened with the prolongation of treatment time. The above results show that UV-B radiation deepened the membrane lipid peroxidation of cabbage, while the absorption of UV flavonoids does not completely alleviate the UV-B damage.