论文部分内容阅读
以热休克抑制受精卵第一次卵裂，进行日本沼虾四倍体诱导实验。热休克温度在３８、３９、４０和４１℃，于产卵２１０～２３０分钟后，处理１～２分钟均可获得四倍体胚胎，４０℃处理１．５分钟，四倍体胚胎诱导率达３６．８％，３８和３９℃处理２分钟所获得的嵌合体胚胎比率大于四倍胚胎比率，而热休克温度４０℃时，四倍体率显著高于嵌合体比率。４１℃处理时由于有丝分裂异常导致胚胎死亡率高，更适宜的处理时间有待进一步研究，以便提高四倍体育成率
Heat shock was applied to inhibit the first cleavage of zygotes, and the tetraploid induction of M. japonicum was carried out. Heat shock temperature at 38, 39, 40 and 41 ℃, 210 to 230 minutes after laying eggs, the treatment of 1 to 2 minutes to obtain tetraploid embryos, 40 ℃ for 1.5 minutes, tetraploid embryos induction rate of up to 36.8%, 38 and 39 ℃ for 2 minutes, the rate of chimeric embryos obtained was more than four times the embryo ratio, while the heat shock temperature was 40 ℃, the tetraploid rate was significantly higher than the chimera ratio. Higher embryo mortality due to mitotic abnormalities at 41 ° C, more suitable treatment time to be further studied in order to increase the quadrupled P.E.