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目的 观察 5 氟尿嘧啶 (5 Fu)前体物质卡培他滨 (Capecitabine)对高转移潜能肝癌LCID2 0生长和转移的抑制作用。方法 用免疫组织化学方法检测 6 1例人肝癌组织中血小板衍化内皮细胞生长因子 (PD ECGF)蛋白水平。 18只裸鼠人肝癌高转移模型LCID2 0于肿瘤种植后第 3天分别采用CAP和 5 Fu治疗。停药后第 3天处死裸鼠 ,测量肿瘤的长短径 ,检测裸鼠肝功能和血浆甲胎蛋白 (AFP)水平 ;苏木素 伊红 (HE)染色检测肺转移 ;原位末端标记 (TUNEL)法检测肝肿瘤组织中的凋亡细胞。结果 6 1例肝癌和癌周组织中PD ECGF蛋白表达阳性率分别为 70 .5 %和 47.5 % ,高TNM分期组肝癌组织中PD ECGF蛋白表达阳性率 (81.1% )明显高于低TNM分期组表达率(5 4.2 % ) ,两者差异有显著性 (P <0 .0 5 )。CAP治疗后 ,裸鼠肝肿瘤体积 (2 40± 119)mm3 明显小于对照组 (4 47± 15 9)mm3 和 5 Fu治疗组 (4 42± 81)mm3(P <0 .0 5 ) ;血浆AFP水平明显下降 (P <0 .0 1) ;肺转移率显著降低 (P <0 .0 1) ;肝肿瘤组织中凋亡指数增大 (P <0 .0 1)。结论 CAP治疗能抑制高转移性人肝癌的生长和转移。
Objective To observe the inhibitory effect of 5 fluorouracil (5 Fu) precursor substance capecitabine on the growth and metastasis of hepatocellular carcinoma LCID2 0 with high metastatic potential. Methods Immunohistochemistry was used to detect platelet-derived endothelial cell growth factor (PD ECGF) protein levels in 61 cases of human hepatocellular carcinoma. 18 human high-metastatic models of liver cancer, nude mice, were treated with CAP and 5 Fu on the third day after tumor implantation. The nude mice were sacrificed on the third day after drug withdrawal. The length and diameter of the tumor were measured, and the levels of hepatic function and plasma alpha-fetoprotein (AFP) in nude mice were measured. Hematoxylin-eosin (HE) staining was used to detect lung metastasis; TUNEL method was used. Detection of apoptotic cells in liver tumor tissue. Results The positive rates of PD ECGF protein expression in 61 cases of hepatocellular carcinoma and pericancerous tissue were 70.5% and 47.5%, respectively. The positive rate of PD ECGF protein expression in hepatocellular carcinoma was significantly higher (81.1%) in the high TNM stage group than in the low TNM stage group. The rate of expression (5 4.2%) was significantly different (P < 0.05). After CAP treatment, the tumor volume of nude mice (2 40 ± 119) mm3 was significantly lower than that of the control group (4 47 ± 15 9) mm3 and 5 Fu treatment group (4 42 ± 81) mm3 (P <0.05); The level of AFP decreased significantly (P <0.01); the rate of lung metastasis decreased significantly (P <0.01); the apoptotic index increased in liver tumors (P <0.01). Conclusion CAP treatment can inhibit the growth and metastasis of highly metastatic human hepatoma.