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目的:观察低温、巴曲酶及巴曲酶复合低温对全脑缺血再灌注损伤的影响。方法:采用蒙古沙土鼠全脑缺血模型。观察缺血10min、再灌20min及60min时,应用巴曲酶、低温及巴曲酶复合低温对沙土鼠脑海马组织超氧化物歧化酶(superoxidedismutase,SOD)、丙二醛的影响。结果:随着缺血时间的延长SOD活性有下降趋势,与对照组比较低温组复灌20,60min分别下降5.85%和8.23%;巴曲酶组复灌20,60min分别下降18.3%和5.12%。低温和巴曲酶均可减轻SOD活性下降(P<0.01);而巴曲酶复合低温组效果更优于单独应用(P<0.05);丙二醛含量随着复灌时间的延长有增高趋势,能降低其生成含量(P<0.01);两者复合应用效果更优。结论:巴曲酶及低温均具有脑保护作用,巴曲酶复合低温可增强脑保护作用。
Objective: To observe the effects of hypothermia, batroxobin and batroxobin on the global cerebral ischemia-reperfusion injury. Methods: Mongolian gerbil model of global ischemia was used. The effects of batroxobin, hypothermia and batroxobin combined with hypothermia on superoxide dismutase (SOD) and malondialdehyde (MDA) in hippocampus of gerbils were observed after ischemia 10 min, reperfusion 20 min and 60 min. Results: Compared with the control group, the activity of SOD in the hypothermia group decreased by 5.85% and 8.23%, respectively, compared with the control group, while that in the batroxobin group decreased by 18.3% and 5.12% . Both low temperature and batroxobin reduced the SOD activity (P <0.01), while Batroxobin combined with low temperature group was more effective than single use (P <0.05). MDA content increased with the extension of reperfusion time , Can reduce its generation content (P <0.01); The combination of the two is better. Conclusion: Both batroxobin and hypothermia have neuroprotective effects. Batroxobin combined with hypothermia can enhance the protective effect of the brain.