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目的比较分析两种不同高脂饲料对大鼠血脂的影响,为建立合理而实用的高脂血症动物模型提供依据。方法 Wistar大鼠30只,按体重随机分为对照组(喂饲基础饲料),高脂配方1组和高脂配方2组,每组各10只,实验期间每周记录一次大鼠体重,每两周检测一次血脂,实验周期为6周。结果高脂饲料诱导6周后,高脂配方1组大鼠的血清总胆固醇(TC)和低密度脂蛋白胆固醇(LDL-C)分别比对照组升高了1.4倍和2.2倍(P<0.01),血清高密度脂蛋白胆固醇(HDL-C)水平比对照组降低了22.2%(P<0.01),血清甘油三酯(TG)水平与对照组无显著差异(P>0.05);高脂配方2组大鼠的血清TC和LDL-C分别比对照组升高了11.5倍和5.7倍(P<0.01),血清TG和HDL-C水平分别比对照组降低了39.7%和34.6%(P<0.01)。结论两种高脂饲料诱导均可建立大鼠高胆固醇血症模型,但均不能显著升高大鼠血清TG水平。
Objective To compare and analyze the effects of two kinds of high-fat diets on blood lipids in rats and provide the basis for establishing a rational and practical animal model of hyperlipidemia. Methods Thirty Wistar rats were randomly divided into control group (fed with basal diet), high fat diet group 1 and high fat diet group 2 with 10 rats in each group. Body weight of rats was recorded every week Two weeks of testing a lipid, the experimental period of 6 weeks. Results After 6 weeks of induction with high-fat diet, the levels of total cholesterol (TC) and low density lipoprotein cholesterol (LDL-C) in high-fat diet group 1 were 1.4 times and 2.2 times higher than those in control group (P <0.01), serum triglyceride (TG) level was not significantly different from that of the control group (P> 0.05). The level of serum high density lipoprotein cholesterol (HDL-C) The levels of TC and LDL-C in the two groups were increased by 11.5 times and 5.7 times (P <0.01), and the serum levels of TG and HDL-C were decreased by 39.7% and 34.6% respectively compared with the control group (P < 0.01). Conclusion Both hypercholesterolemic models can be established by both high-fat diets, but none of them can significantly increase serum TG levels.