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目的探讨白细胞介素-12p40(IL-12p40)和IL-12p70在不同病原菌致小鼠血流感染模型中的动力学变化及意义。方法建立金黄色葡萄球菌、粪肠球菌、大肠埃希菌和肺炎克雷伯菌标准菌株的CD-1(ICR)小鼠血流感染模型并评价;采用蛋白液相芯片检测技术测定各实验组与磷酸缓冲溶液(PBS)的对照组0.5、1、3、6、12、24和48h的IL-12p40和IL-12p70浓度。结果金黄色葡萄球菌组与对照组相比,IL-12p40的浓度稍升高,而粪肠球菌组、大肠埃希菌组和肺炎克雷伯菌组感染后1h即明显上升,3h时达峰值,分别为(216.36±50.63)pg/mL、(118.32±9.98)pg/mL和(129.19±27.11)pg/mL。感染12h后仍维持较高水平,与对照组相比差异有统计学意义(P<0.05);金黄色葡萄球菌组IL-12p70浓度在感染后6h时明显升高且达峰值,为(389.31±118.07)pg/ml;粪肠球菌组IL-12p70浓度在感染后3h明显升高,与对照组相比差异有统计学意义(P<0.05),大肠埃希菌组与肺炎克雷伯菌组IL-12p70浓度于感染后3h达峰值,分别为(42.20±3.54)pg/mL和(62.80±8.37)pg/mL但升高幅度显著低于金黄色葡萄球菌组和粪肠球菌组(P<0.05)。结论 IL-12p40和IL-12p70在四组单病原菌性血流感染模型早期均明显升高,感染金黄色葡萄球菌或粪肠球菌后IL-12p70浓度的升高程度明显高于大肠埃希菌或肺炎克雷伯菌感染。联合检测IL-12p40和IL-12p70等指标对区分革兰阴阳性菌感染有一定价值。
Objective To investigate the dynamic changes and significance of interleukin-12p40 (IL-12p40) and IL-12p70 in blood-infected mice induced by different pathogenic bacteria. Methods The CD-1 (ICR) mice bloodstream infection model of Staphylococcus aureus, Enterococcus faecalis, Escherichia coli and Klebsiella pneumoniae was established and evaluated. The protein microarray IL-12p40 and IL-12p70 concentrations at 0.5, 1, 3, 6, 12, 24 and 48h with phosphate buffered saline (PBS) Results Compared with the control group, the concentration of IL-12p40 slightly increased in Staphylococcus aureus group, but it increased significantly at 1h after Enterococcus faecalis group, Escherichia coli group and Klebsiella pneumoniae group, and peaked at 3h (216.36 ± 50.63) pg / mL, (118.32 ± 9.98) pg / mL and (129.19 ± 27.11) pg / mL, respectively). The level of IL-12p70 in Staphylococcus aureus group was significantly higher and peaked at 6h after infection (389.31 ± 118.07) pg / ml. The concentration of IL-12p70 in Enterococcus faecalis group was significantly increased at 3h after infection, which was significantly different from the control group (P <0.05). The Escherichia coli group and Klebsiella pneumoniae group The concentration of IL-12p70 peaked at 3h after infection (42.20 ± 3.54 pg / mL and 62.80 ± 8.37 pg / mL, respectively, but the increase was significantly lower than that of Staphylococcus aureus and Enterococcus faecalis (P < 0.05). Conclusions IL-12p40 and IL-12p70 were significantly increased in the four groups of single-pathogenic bloodstream infection in the early stage. After the infection of S. aureus or E. faecalis, the increase of IL-12p70 level was significantly higher than that of Escherichia coli or Escherichia coli Klebsiella pneumoniae infection. Joint detection of IL-12p40 and IL-12p70 and other indicators on the identification of gram-positive and negative bacteria have some value.