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目的本研究旨在结合腹膜后淋巴结切除术，探讨癌蛋白ｎｍ２３Ｈ１，ｐ１８５，ｐ２１，ｐ５３在卵巢癌中的表达与卵巢癌腹膜后淋巴转移（ＬＮＭ）的关系。方法采用免疫组化方法测定石腊包埋标本中ｎｍ２３Ｈ１，ｐ１８５，ｐ２１和ｐ５３的表达，单因素、多因素分析它们的表达与淋巴结转移关系。结果在３１例（３１．６％）患者中ｎｍ２３Ｈ１蛋白表达阳性；ｐ２１的表达与组织分化程度及残留癌相关。病理证实４９例（５５．１％）有淋巴结转移；ｎｍ２３Ｈ１蛋白表达阳性的ＬＮＭ率低（３５．７％ｖｓ６３．９％，Ｐ＝０．０１２）；ｐ２１表达与ＬＮＭ正相关（Ｐ＜０．００１）；ｐ１８５（～）病例ＬＮＭ率（６４．４％）高于ｐ１８５（＋）或ｐ１８５（－）的转移率（４５．９％），但差异无统计学意义；ｐ５３表达与ＬＮＭ无关。与ＬＮＭ相关的临床因素是伴有腹水、组织分化不良、残留灶和分期晚。经Ｌｏｇｉｓｔｉｃ回归多因素分析，影响淋巴结转移的独立危险因素是ｎｍ２３Ｈ１蛋白阴性表达，ｐ２１的阳性表达、有残留灶和分化差。结论在卵巢恶性肿瘤中，ｎｍ２３Ｈ１蛋白和ｐ２１的表达对腹膜后淋巴结转移有独立的联合作用，而ｐ１８５和ｐ５３的表达则无关。
Objective To investigate the relationship between the expression of oncoproteins nm23H1, p185, p21 and p53 in ovarian cancer and retroperitoneal lymphatic metastasis (LNM) in ovarian cancer in combination with retroperitoneal lymph node dissection. Methods Immunohistochemistry was used to detect the expression of nm23H1, p185, p21 and p53 in paraffin embedded specimens. The relationship between them was analyzed by multivariate and multi-factor analysis. Results The positive expression of nm23H1 was found in 31 patients (31.6%). The expression of p21 was correlated with the degree of tissue differentiation and residual cancer. The lymph node metastasis was confirmed in 49 cases (55.1%) with pathological examination. The LNM positive rate of nm23H1 protein was low (35.7% vs 63.9%, P = 0.012). The positive correlation between p21 expression and LNM was found (P <0.05). 001). The LNM rate (64.4%) in p185 ( ~ ) cases was higher than that in p185 (+) or p185 (-) cases (45.9%), but the difference was not statistically significant. Nothing to do Clinical factors associated with LNM are associated with ascites, poor tissue differentiation, residual foci and staging. Logistic regression multivariate analysis showed that the independent risk factors of lymph node metastasis were negative expression of nm23H1 protein, positive expression of p21, residual tumor and poor differentiation. Conclusion The expression of nm23H1 protein and p21 in ovarian cancer has an independent effect on the metastasis of retroperitoneal lymph node. However, the expression of p185 and p53 has no correlation.