论文部分内容阅读
目的 探讨p5 3和Bcl- 2蛋白在实验性急性心肌梗死 (AMI)心肌细胞凋亡中的作用。方法 用TUNEL法检测 48只兔AMI模型梗死区的心肌细胞凋亡 ,用免疫组化法检测p5 3和Bcl- 2蛋白在心肌细胞中的表达水平 ,并探讨它们之间的相互关系。结果 TUNEL法发现兔梗死区心肌细胞凋亡千分率在冠脉结扎前 (0h)和结扎后 1、2、3、4、6、8、12h的改变如下 :0h组 (31 0 0± 14 0 0‰ )和 12h组 (6 8 0 0± 31 72‰ ) <1h组 (142 33± 5 3 5 2‰ ) <2h组 (370 83± 6 6 0 1‰ )和 8h组 (32 1 17± 86 43‰ ) <3h组 (5 86 83± 5 5 0 2‰ ) <4h组 (836 17± 43 73‰ ) (P均 <0 0 5 ) ,即急性心梗心肌细胞凋亡数在冠脉结扎后 1h时开始逐渐增多 ,到 4h时达高峰 ,其后逐渐下降 ,到 12h时已逐渐降至正常 ;阳性表达的p5 3蛋白积聚于胞核内 ,阳性心肌细胞千分率在冠脉未结扎 (0h)组 (4 5± 4 14 )和结扎后 1h组 (37 33± 35 43‰ )、2h组 (71 33± 5 4 93‰ )、8h组 (76 6 0±45 31‰ )、12h组 (6 5 0± 4 32‰ ) (P均 <0 0 5 ) ,而其它各组之间相互比较则无统计学上的差异 (P均 >0 0 5 ) ;阳性的Bcl- 2蛋白积聚于胞浆中 ,阳性心肌细胞千分率在冠脉结扎后 3h组 (41 0 0± 2 1 6 0‰ )、4h组 (6 83± 5 34‰ )和 6
Objective To investigate the role of p53 and Bcl-2 in cardiomyocyte apoptosis in experimental acute myocardial infarction (AMI). Methods TUNEL method was used to detect the apoptosis of myocardial cells in the infarct area of 48 rabbits with AMI. The expressions of p53 and Bcl-2 protein in cardiomyocytes were detected by immunohistochemistry and the relationship between them was explored. Results TUNEL assay showed that the percentage of cardiomyocyte apoptosis in rabbit infarction zone before coronary artery occlusion (0 h) and at 1, 2, 3, 4, 6, 8 and 12 h after ligation were as follows: 0h group (31 0 ± 14 (0%) and 12h group (6800 ± 3172% o) <1h group (14233 ± 5322% o) <2h group (37083 ± 6601% o) and 8h group ± 86 43 ‰) (3 863 ± 5 5 0 2 ‰) <4h group (836 17 ± 43 73 ‰) (P all <0.05), that is, the number of apoptotic cardiomyocytes in acute myocardial infarction Pulse ligation began to gradually increase at 1h, reached its peak at 4h, and then gradually decreased to 12h when it gradually reduced to normal; positive expression of p5 3 protein accumulated in the nucleus, the positive rate of myocardial cells in the coronary artery (45 ± 4 14) and 1 h after ligation (37 33 ± 35 43 ‰), 2 h (71 33 ± 5 4 93 ‰) and 8 h (76 6 ± 45 31 ‰) , And 12h group (65 0 ± 4 32 ‰) (P <0.05), while there was no significant difference among the other groups (P> 0.05). Positive Bcl-2 Protein accumulation in the cytoplasm, positive myocardial cell percentage in the 3h after coronary ligation (41 0 0 ± 2 1 6 0 ‰), 4h group (6 83 ± 5 34 ‰) and 6