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Objective To explore the expression of etheràgo-go1(Eag1)in human osteosarcoma and its molecular mechanisms of regulating the malignant phenotype of osteosarcoma.Methods The expression levels of Eag1 in osteosarcoma cell lines and human osteosarcoma tissues were detected by reverse transcription polymerase chain reaction(RT-PCR),western blot analysis and immunohistochemistry.The small interfering RNA(siRNA)was used
Objective To explore the expression of etheràgo-go1 (Eag1) in human osteosarcoma and its molecular mechanisms of regulating the malignant phenotype of osteosarcoma. Methods The expression levels of Eag1 in osteosarcoma cell lines and human osteosarcoma tissues were detected by reverse transcription polymerase chain reaction ( RT-PCR), western blot analysis and immunohistochemistry. The small interfering RNA (siRNA) was used