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目的探讨E26转录因子-1(E26ts-1)、基质金属蛋白酶-1(MMP-1)及其抑制剂(TIMP-1)在脉络膜黑色素瘤中的表达及其与肿瘤浸润及转移的关系。方法以免疫组织化学法检测78例葡萄膜黑色素瘤患者中的E26ts-1,MMP-1和TIMP-1的表达,并按肿瘤细胞的形态进行分型:梭型细胞型,类上皮细胞型和混合细胞型。进行临床随访,计算平均生存时间,以SPSS10.0统计软件包进行统计学处理。结果78例脉络膜黑色素瘤患者中,梭型细胞型21例,类上皮细胞型34例,混合细胞占23例。TIMP-1呈低表达,E26Ts-1和MMP-1在三种类型的脉络膜黑色素瘤中均有表达;表达强度为梭型细胞型、混合细胞型和类上皮细胞型依次递增。共回访37例患者,其中梭型细胞型18例,平均生存时间为(78.33±24.69)个月;混合细胞型10例,平均生存时间(61.44±20.46)个月;类上皮细胞型9例,平均生存时间(36.76±12.19)个月。患者生存时间与E26ts-1及MMP-1的表达强度呈负相关(P<0.01)。结论E26Ts-1和MMP-1的高表达及TIMP-1的低表达可能与脉络膜黑色素瘤的转移浸润有关。
Objective To investigate the expression of E26ts-1, MMP-1 and TIMP-1 in choroidal melanoma and its relationship with tumor invasion and metastasis. Methods The expressions of E26ts-1, MMP-1 and TIMP-1 in 78 patients with uveal melanoma were detected by immunohistochemistry and classified according to the morphology of tumor cells: spindle cell type, epithelial cell type and Mixed cell type. Clinical follow-up, calculate the average survival time, statistical package SPSS10.0 for statistical analysis. Results Among the 78 patients with choroidal melanoma, 21 were spindle-type cells, 34 were epithelial-like cells and 23 were mixed cells. TIMP-1 was low expression, E26Ts-1 and MMP-1 were expressed in all three types of choroidal melanoma; the expression intensity was spindle cell type, mixed cell type and epithelioid cell type in increasing order. A total of 37 patients were retrospectively analyzed. Among them, 18 were spindle cell type with an average survival time of (78.33 ± 24.69) months; 10 were mixed cell type with an average survival time of (61.44 ± 20.46) months; 9 were epithelial cell type The average survival time (36.76 ± 12.19) months. The survival time was negatively correlated with the expression of E26ts-1 and MMP-1 (P <0.01). Conclusion The high expression of E26Ts-1 and MMP-1 and the low expression of TIMP-1 may be related to the metastasis and invasion of choroidal melanoma.