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应用SDS-PAGE和免疫印渍试验(EITB),证明体外培养的细粒棘球蚴生发细胞和囊液含有特异性抗原,分子量为52kDa和38kDa,而囊壁则含有此2种抗原。生发细胞的52kDa抗原可识别感染小鼠和包虫病患者血清的特异性抗体,而对囊虫病人和正常人血清则无反应条带。
SDS-PAGE and immunoblotting test (EITB) showed that the germinal cells and cyst fluid of Echinococcus granulosus cultured in vitro contained specific antigens with molecular weights of 52 kDa and 38 kDa, while the cytoplasm contained these two antigens. The 52kDa antigen in germinal cells recognizes specific antibodies in the sera from infected mice and hydatid patients, but no response to cysticercosis and normal human sera.