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为调查高寒牧区引起奶牛乳房炎的凝固酶阴性致病葡萄球菌流行现状及耐药情况,本研究对呼伦贝尔地区3个规模化奶牛养殖场采用加州乳房炎测试法(CMT)进行高体细胞牛脂病检测,并采集330份牛奶样品,应用PCR方法、脉冲场凝胶电泳(PFGE)分型和药敏试验等对样品中的葡萄球菌进行分离鉴定和耐药性分析。结果显示,该地区隐性乳房炎发病率达47.8%,共分离和鉴定出9种葡萄球菌60株(34.5%);60株葡萄球菌分为41个克隆谱型;60株葡萄球菌对青霉素和林可霉素的耐药率较高,分别为40.0%(24/60)和50.0%(30/60),表明这两种药是治疗乳房炎常用药物。本研究为高寒地区致病葡萄球菌引起的奶牛乳房炎临床合理用药及对病原菌的防控提供实验依据和指导。
In order to investigate the prevalence and drug resistance of coagulase-negative staphylococcus causing dairy mastitis in alpine pastoral areas, three large-scale dairy cattle farms in Hulunbeier were used to test the mastocytosis Samples of 330 samples of milk samples were collected and tested. Staphylococcus was isolated and identified and analyzed for drug resistance by PCR, PFGE typing and drug susceptibility testing. The results showed that the prevalence rate of latent mastitis in this area was 47.8%, 60 strains (9.5%) of 9 kinds of staphylococcus were isolated and identified, 60 strains of staphylococci were divided into 41 clonotypes, 60 strains of staphylococci were sensitive to penicillin and The higher rates of resistance to lincomycin were 40.0% (24/60) and 50.0% (30/60), respectively, indicating that these two drugs are commonly used in the treatment of mastitis. This study provides experimental evidence and guidance for the clinical rational use of the drug and the prevention and control of pathogenic bacteria caused by Staphylococcus aureus in the alpine region.