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本研究用RIA测大鼠在几种不同条件下,血浆、垂体和下丘脑中β内啡肽免疫活性物质(IR-β-EP)的含量以观察其差异。结果显示:大鼠在近似常氧的高压氮氧中与在常压空气中暴露相比,垂体IR-β-EP含量显著较低。血浆IR-β-EP含量显著较高,但下丘脑IR-β-EP含量几乎没有差异;大鼠在高压氧中与在近似常氧的高压氮氧中暴露相比,垂体IR-β-EP含量亦显著较低,血浆IR-β-EP含量亦显著较高,下丘脑IR-β-EP含量也有一定程度的增高(P>0.05)。动物发生惊厥时,上述变化更趋显著,下丘脑IR-β-EP含量亦有统计学上的显著增高(P<0.05)。此外,还观察纳洛酮和β内啡肽(β-EP)抗血清对大鼠高压氧(HBO)露露时的惊厥始发时间(ITC)和严重程度(SOC)以及出舱时死亡率(MOS)的影响。结果提示:β-EP可能参与氧惊厥的发作过程,它可能是导致氧惊厥的内源性物质之一。
In this study, RIA was used to measure the content of β-endorphin immunoreactive substance (IR-β-EP) in plasma, pituitary and hypothalamus in different conditions. The results showed that the pituitary IR-β-EP content was significantly lower in rats exposed to normoxia than those exposed to atmospheric air. Plasma IR-β-EP content was significantly higher, but there was almost no difference in hypothalamic IR-β-EP content. Compared with exposure to hyperbaric oxygen in near-normoxia, The content of IR-β-EP in plasma was also significantly higher, and the content of IR-β-EP in hypothalamus was also increased to a certain extent (P> 0.05). The above changes were more pronounced in animals with convulsion, and the content of IR-β-EP in hypothalamus also increased significantly (P <0.05). In addition, the seizure onset time (ITC) and severity (SOC) of naloxone and beta-endorphin (β-EP) antisera on rats exposed to hyperbaric oxygen (HBO) (MOS) effects. The results suggest that β-EP may be involved in the process of onset of oxygen convulsions, which may be one of the endogenous substances that cause oxygen convulsion.