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目的探讨miRNA-26a在良、恶性腹水鉴别诊断中的意义。方法收集北京大学深圳医院2011年9月～2014年3月门诊及住院患者60例,包括20例肝硬化腹水患者(肝硬化腹水组),20例结核性腹水患者(结核性腹水组),20例恶性腹水患者(恶性腹水组)。采用实时定量PCR(qRT-PCR)方法检测并比较各组血清miRNA-26a、甲胎蛋白(AFP)、糖类抗原125(CA125)的表达。结果恶性腹水组血清CA125表达高于结核性腹水组和肝硬化腹水组,但差异无统计学意义(P>0.05)。AFP、miRNA-26a在结核性腹水组及肝硬化腹水组表达差异无统计学意义(P>0.05);AFP和miRNA-26a在恶性腹水组患者血清表达明显高于结核性腹水组和肝硬化腹水组,差异有统计学意义(P<0.05)。结论 miRNA-26a水平将来有可能有助于良、恶性腹水的鉴别诊断。
Objective To investigate the significance of miRNA-26a in the differential diagnosis of benign and malignant ascites. Methods Sixty outpatients and inpatients from Peking University Shenzhen Hospital from September 2011 to March 2014 were collected, including 20 patients with cirrhosis and ascites (cirrhosis ascites group), 20 patients with tuberculous ascites (tuberculous ascites group), 20 Cases of malignant ascites patients (malignant ascites group). Real-time quantitative PCR (qRT-PCR) was used to detect and compare the expression of miRNA-26a, AFP and CA125 in each group. Results The serum CA125 level in malignant ascites group was higher than that in tuberculous ascites group and cirrhosis group, but the difference was not statistically significant (P> 0.05). The expression of AFP and miRNA-26a in tuberculous ascites group and cirrhosis group had no significant difference (P> 0.05); AFP and miRNA-26a in malignant ascites group was significantly higher than that in tuberculous ascites group and cirrhosis ascites group Group, the difference was statistically significant (P <0.05). Conclusion The miRNA-26a level in the future may contribute to the differential diagnosis of benign and malignant ascites.