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目的探讨猪胰脏和胰酶两种消化方式制备的消化液对鼠疫菌生长效果观察,为鼠疫赫氏培养基制作提供借鉴。方法采用同种方法不同消化方式制成两种消化液,对其进行外观检查和氨基氮含量测定及鼠疫菌活菌计数观察。结果两种消化方式制备的消化液外观无区别,氨基氮含量均为800 mg/L以上,鼠疫菌生长试验经统计分析无显著性差异(P>0.05)。结论猪胰脏和胰酶均能用于鼠疫赫氏培养基制作,可视不同工作需求选择使用。
Objective To investigate the effect of digestive juice prepared by two kinds of digestion methods of porcine pancreas and pancreatin on the growth of Yersinia pestis and provide reference for the production of Plaguehorse culture medium. Methods Two kinds of digestive juice were made by the same method and different digestion methods. The appearance and amino nitrogen contents of the two digestive juice were measured and the viable counts of Yersinia pestis were observed. Results There was no difference in the appearance of digestive juice prepared by the two digestion methods. The content of amino nitrogen was above 800 mg / L. There was no significant difference in the growth of Yersinia pestis between the two groups (P> 0.05). Conclusion Porcine pancreas and trypsin can be used for the production of Plague Hemischine, which can be selected according to different work needs.