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本文通过大鼠血红蛋白耗竭-恢复试验测定了分离大豆蛋白(ISP)、浓缩大豆蛋白(CSP)、浸泡和发芽大豆粉以及强化含硫氯基酸分离大豆蛋白铁的生物利用率。结果饲以ISP和CSP饲料的贫血大鼠其Hb均未恢复到耗竭试验前的正常水平(12.4g±1.56g%)。而后3种饲料则可使铁的相对生物利用率提高到93%~98%。本文还用体外方法模仿胃肠消化条件测定了几种大豆制品的可利用铁,与生物利用率测定结果基本一致。
In this study, the rat hemoglobin depletion-recovery test was used to determine the soy protein isolate (ISP), concentrated soy protein (CSP), soaked and germinated soy flour, and the bioavailability of sulfur-containing chloric acid to separate soy protein iron. Results The Hb of the anemic rats fed with ISP and CSP diet did not return to the normal level before depletion test (12.4g ± 1.56g%). Then the three kinds of feed can increase the relative bioavailability of iron to 93% to 98%. This article also used in vitro methods to imitate the gastrointestinal digestive conditions to determine the available iron of several soybean products, and is basically consistent with the results of bioavailability measurement.