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目的探讨人类乳头瘤病毒ＨＰＶ１６，１８型感染与胃癌发生的关系．方法采用ＨＰＶ１６Ｅ６和ＨＰＶ１８Ｅ６／Ｅ７特异性引物，通过多聚酶链反应（ＰＣＲ），检测３７例新鲜胃癌组织标本及２０例新鲜癌旁正常胃粘膜标本中的ＨＰＶＤＮＡ．结果新鲜胃癌组织３７例中ＨＰＶ１６，１８型ＤＮＡ检出率分别为２１６％和５４％．新鲜癌旁正常胃粘膜标本２０例中检出率为０．ＨＰＶ１６型ＤＮＡ在胃癌和癌旁正常胃粘膜中检出率存在显著性差异（Ｐ＜００５），而ＨＰＶ１８则没有显著性差异（Ｐ＞００５）．新鲜胃癌组织３７例中ＨＰＶ１６，１８型ＤＮＡ总检出率为２７０％，２０例新鲜癌旁正常胃粘膜标本中总检出率为０，两者有显著性差异（Ｐ＜００５）．新鲜胃癌组织３７例中ＨＰＶ１６，１８型双重感染检出率为０．ＨＰＶ１６和（或）ＨＰＶ１８型ＤＮＡ的检出率在不同分化程度的胃癌中无显著性差异，但ＨＰＶ１６型ＤＮＡ在不同部位的胃癌中检出率却有显著性差异，以贲门部胃癌检出率最高．结论高危类ＨＰＶ１６型感染可能与胃癌发生有关，ＨＰＶ１６型病毒癌基因可能在胃癌形成过程中起一定作用
Objective To investigate the relationship between human papillomavirus HPV16,18 infection and gastric cancer. Methods HPV DNA was detected in 37 specimens of fresh gastric cancer tissues and 20 specimens of fresh normal gastric mucosa by polymerase chain reaction (PCR) using HPV16E6 and HPV18E6/E7 specific primers. Results The detection rate of HPV16, 18 DNA in fresh gastric cancer tissues was 216% and 5.4%, respectively. The detection rate was 0 in 20 cases of normal gastric mucosa adjacent to fresh cancer. There was a significant difference in the detection rate of HPV16 DNA in gastric cancer and adjacent normal gastric mucosa (P<005), while there was no significant difference in HPV18 (P>005). The total detection rate of HPV16,18 DNA was 37.0% in fresh gastric cancer tissues. The total detection rate of 20 samples of fresh normal gastric mucosa was 0. There was a significant difference between them (P<005). ). The detection rate of double-infection of HPV16,18 in 37 cases of fresh gastric cancer was 0. There was no significant difference in the detection rate of HPV16 and/or HPV18 DNA among gastric cancers with different degrees of differentiation. However, there was a significant difference in the detection rate of HPV16 DNA in different parts of gastric cancer. highest. Conclusion High-risk HPV16 infection may be related to gastric cancer. HPV16 oncogene may play a role in the formation of gastric cancer.