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目的:建立测定新舒通胶囊中血竭素与亚油酸含量的高效液相色谱法。方法:采用高效液相色谱法,菲罗门Kinetex C18色谱柱(100 mm×4.6 mm,2.6μm),以乙腈为流动相A,0.1%磷酸溶液为流动相B,进行梯度洗脱,检测波长为210 nm,柱温为35℃,流速为1.0 ml·min-1。结果:血竭素进样量在79.22×10-6~15.84×10-4mg,亚油酸在89.56×10-5~17.91×10-3mg范围内与峰面积呈良好线性关系(r1=0.999 9,血竭素;r2=0.999 9,亚油酸),血竭素加样回收率为99.9%、RSD为1.0%(n=6),亚油酸加样回收率为98.3%、RSD为1.4%(n=6)。结论:该方法快速、灵敏、准确,可作为新舒通胶囊的含量测定方法。
Objective: To establish a HPLC method for determination of dracorhodin and linoleic acid in Xin Shutong Capsules. METHODS: HPLC was performed on a Phenomenex Kinetex C18 column (100 mm × 4.6 mm, 2.6 μm) using acetonitrile as mobile phase A and 0.1% phosphoric acid as mobile phase B. The gradient elution was carried out. The detection wavelength Was 210 nm, the column temperature was 35 ℃, the flow rate was 1.0 ml · min-1. Results: Doxorubicin injection volume was 79.22 × 10-6 ~ 15.84 × 10-4mg, linoleic acid showed a good linear relationship with the peak area in the range of 89.56 × 10-5 ~ 17.91 × 10-3mg (r1 = 0.999 9 , Dracorhodin; r2 = 0.999 9, linoleic acid), the recovery of DRX was 99.9%, the RSD was 1.0% (n = 6), the recovery of linoleic acid was 98.3% % (n = 6). Conclusion: The method is rapid, sensitive and accurate and can be used as a method for the determination of Xin Shutong capsule.