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本文作者观察了不同年龄和不同性别人群其泌尿道上皮细胞对致病性大肠杆茵的粘附能力。方法1.受检者83例,平均年龄84.5岁(71～97),其中62例无菌尿症(男26例,女36例),21例女性无症状菌尿症(含菌量≥10~5/ml个细菌)。对照者40例(男21,女19),平均年龄26.2岁(22～40)无菌尿症及泌尿道感染史。 2.取受检者晨尿50ml于试管中,4℃,250×g离心15分钟,去上层液,留下1～2毫升转入厚壁试管,加pH7.2缓冲液至20毫升,再离心,去上层液留下3毫升,调整细胞浓度至5×10~4/ml。
The authors observed the adhesion of urothelial cells to pathogenic E. coli in different age and gender groups. Methods A total of 83 subjects with an average age of 84.5 years (71-97 years), including 62 cases of sterile urine disease (26 males and 36 females) and 21 women with asymptomatic bacteriuria ~ 5 / ml bacteria). Control 40 cases (male 21, female 19), the average age of 26.2 years (22 to 40), a history of aseptic urinary tract infection and urinary tract infections. Take the morning urine 50ml in the test tube, centrifuge at 250 × g for 15 minutes at 4 ℃ to the supernatant, leaving 1 ~ 2 ml transferred to thick-walled tubes, add buffer pH7.2 to 20 ml, then Centrifugation, the supernatant to leave 3 ml, adjust the cell concentration to 5 × 10 ~ 4 / ml.