论文部分内容阅读
目的研究树突状细胞对小剂量化疗疗效的影响,探讨小剂量化疗的可能机制。方法以615小鼠的前胃癌细胞株(MFC)造模,在体外用rmGM-CSF和rm IL-4从荷瘤小鼠骨髓细胞分化、诱导未成熟树突状细胞。分为4组:小剂量化疗组、树突状细胞组、小剂量化疗+树突状细胞组、对照组,以BAX试剂盒检测肿瘤凋亡情况。在瘤体内注射树突状细胞,计算抑瘤率、特异性细胞毒性T淋巴细胞(CTL)的增殖及其对肿瘤细胞的特异性杀伤作用。结果小剂量化疗能诱导肿瘤细胞凋亡,BAX基因产物表达增多。注射侧抑瘤率小剂量化疗+DC组、小剂量化疗组、DC组分别为100%、67.22%和57.98%。对侧抑瘤率小剂量化疗+DC组、小剂量化疗组、DC组分别为87.58%、59.69%和48.24%。体内凋亡肿瘤细胞致敏的DC能显著刺激T淋巴细胞增殖,其诱导的CTL对MFC有显著的杀伤作用,在效靶比为40∶1、20∶1、10∶1和5∶1时72 h杀伤率分别为87.64%、70.32%、34.63%和13.87%。并能特异性杀伤小鼠前胃癌细胞MFC(P<0.01)。结论小剂量化疗的机制与肿瘤细胞凋亡及免疫促进有一定的相关。
Objective To study the effect of dendritic cells on the efficacy of low-dose chemotherapy and to explore the possible mechanism of low-dose chemotherapy. METHODS: A 615 human gastric cancer cell line (MFC) was modeled and differentiated from tumor-bearing mouse bone marrow cells with rmGM-CSF and rm IL-4 in vitro to induce immature dendritic cells. Divided into 4 groups: low-dose chemotherapy group, dendritic cell group, low-dose chemotherapy + dendritic cell group, control group, with BAX kit detection of tumor apoptosis. Dendritic cells were injected into the tumor to calculate the tumor inhibition rate, the proliferation of specific cytotoxic T lymphocytes (CTLs) and their specific killing effect on tumor cells. Results Low-dose chemotherapy can induce tumor cell apoptosis, BAX gene product expression increased. Injecting side of tumor inhibition rate of low-dose chemotherapy + DC group, low-dose chemotherapy group, DC group were 100%, 67.22% and 57.98%. Contralateral tumor inhibition rate of low-dose chemotherapy + DC group, low-dose chemotherapy group, DC group were 87.58%, 59.69% and 48.24%. In vivo DCs primed by apoptotic tumor cells could significantly stimulate the proliferation of T lymphocytes. CTLs induced by them induced significant cytotoxicity on MFC. When the target ratios were 40: 1, 20:1, 10:1 and 5:1 The killing rates at 72 h were 87.64%, 70.32%, 34.63% and 13.87% respectively. And can specifically kill mouse gastric cancer cells MFC (P <0.01). Conclusion The mechanism of low-dose chemotherapy is related to tumor cell apoptosis and immune promotion.