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输精管结扎术后睾丸组织学的改变,文献报道不够一致.我们应用光镜和电镜检查了6例输精管结扎术后睾丸活检组织,现将观察结果报道于下.材料和方法6例输精管结扎术后患者,年龄30～38岁,结扎时间1～8年.5例要求复育作输精管吻合术,1例因睾丸鞘膜积液手术.均取左侧睾丸组织活检.标本置于4℃3%戊二醛磷酸缓冲液固定,1%锇酸后固定,环氧树脂618包埋.半薄切片,HE染色.用目镜测微尺在低倍镜(10×10)下每例连续测10个曲细精管直径,求其平均值;在油镜(10×100)下测10个曲细精管界膜的厚度,亦求其平均值.定位后超薄切片,醋酸铀、枸椽酸铅染色,H-600电镜观察.
Vasectomy after testicular histology changes reported in the literature is not consistent enough.We applied light microscopy and electron microscopy examination of 6 cases of vasculitis after testicular biopsy tissue, the results are reported in the next.Materials and methods 6 cases of vasectomy Patients, aged 30 to 38 years old, ligation time of 1 to 8 years .5 cases require re-vasospasm anastomosis, 1 case of hydrocele due to testis .Left testicular biopsies were taken specimens were placed in 4 ℃ 3% Fixed with glutaraldehyde phosphate buffer, fixed with 1% osmium tetroxide, embedded in epoxy resin 618. Semi-thin sections were stained with hematoxylin and eosin (10 × 10) The mean diameter of seminiferous tubules was determined and the average thickness of ten seminiferous tubules was measured under an oil microscope (10 × 100). After positioning, ultrathin sections, uranyl acetate, citric acid Lead staining, H-600 electron microscopy.