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目的 :本研究旨在了解目前临床乙肝检测方法的变化,并对乙型肝炎抗-HBc Ig Ml:1000检测的性能做出评价。方法 :双桥区内医疗机构的临床医生在接诊时发现乙肝病例,通过对病人进行询问,并结合HBV标志物及ALT检测,对符合乙肝病例报告要求的,完成诊断后填写传染病报告卡,同时对每例病例采集5ml血标本,分离血清后分装A、B两管,A管(约l.0ml)用于县级疾控中心进行抗-HBc Ig Ml:1000检测,B管(约l.0ml)备用复核检测。结果 :由于试剂要求将血清稀释1000倍后检测,使原本简单的ELISA实验操作多一道稀释程序,增加了出现人为误差的几率。结论 :对检测质量的提高和优秀的检测系统在临床的推广应用非常重要,对比分析1:1000稀释和不做稀释的区别。
OBJECTIVE: This study aimed to understand the current changes in clinical detection of hepatitis B, and hepatitis B anti-HBc Ig Ml: 1000 to evaluate the performance evaluation. Methods: Clinicians in medical institutions in Shuangqiao District found cases of hepatitis B at the time of admissions. Through inquiring of patients and combined with HBV markers and ALT tests, the report of hepatitis B cases was completed and the report of infectious diseases was completed At the same time, 5ml blood samples were collected for each case, and the serum was separated and divided into A and B tubes. A tube (about 1.0ml) was used in the county CDC to detect anti-HBc Ig Ml: 1000, About l.0ml) backup review test. Results: Since the reagents required to dilute the serum 1000-fold after the test, adding a dilution procedure to a simple ELISA experiment increased the chances of human error. Conclusion: It is very important to improve the quality of detection and excellent detection system in the clinical application. Compare the difference between 1: 1000 dilution and no dilution.