Storage Stability in Reversion Mutation of a Rice Line Devoid of LOX-1, 2 Acquired by Ion Beam Irrad

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The effect of absence of lipoxygenase isoenzyme(LOX)on storage stability wasinvestigated.Rice mutant 1297 without lipoxygenase isoenzyme-1(LOX-1)or lipoxygenaseisoenzyme-2(LOX-2)generated by ion beam irradiation from Wanjian2090 and reversion mutantRM1297 with LOX-1 and LOX-2 were subjected to an accelerated-aging experiment.Shanyou63(with LOX-1 and LOX-2)served as control.Results showed that the germination and dehydro-genase activity decreased while the electrical conductivity and free fatty acid content increased inall varieties with accelerated aging.In 1297 that lacked LOX-1 and 2,there were slight changes ingermination,dehydrogenase activity,membrane permeability and free fatty acid content duringthe thirty-day accelerated-aging experiment.But in varieties with LOX-1 and LOX-2,significantchanges were observed,suggesting that LOX-1,2 might be a definite factor which influenced seedlifespan.This study also indicates that ion beam irradiation may be used as mutagen to generatemutant and reversion mutants for biological study and could become a new direction in ion beamapplication. The effect of absence of lipoxygenase isoenzyme (LOX) on storage stability was investigated. Rice mutant 1297 without lipoxygenase isoenzyme-1 (LOX-1) or lipoxygenase isoenzyme-2 (LOX-2) generated by ion beam irradiation from Wanjian2090 and reversion mutantRM1297 with LOX- 1 and LOX-2 were subjected to an accelerated-aging experiment. Shanyou 63 (with LOX-1 and LOX-2) served as control. Results showed that the germination and dehydro-genase activity decreased while the electrical conductivity and free fatty acid content increased inall varieties with accelerated aging. In 1297 that lacked LOX-1 and 2, there were slight changes ingermination, dehydrogenase activity, membrane permeability and free fatty acid content during the thirty-day accelerated-aging experiment. But in varieties with LOX-1 and LOX -2, significantchanges were observed, suggesting that LOX-1,2 might be a definite factor which influenced seedlifespan. This study also indicates that ion beam irradiation may be used as mutagen to generatemutant and reversion mutants for biological study and could become a new direction in ion beamapplication.
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