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目的探讨内皮型一氧化氮合酶(eNOS)在小鼠单侧输尿管梗阻(UUO)模型肾组织中的变化及其与肾间质纤维化的关系。方法 48只小鼠随机均分为UUO模型组和假手术组,每组术后第1、3、7、14天分别处死6只小鼠,观察肾组织的病理改变,检测eNOS和结缔组织生长因子(CTGF)表达,计算管周毛细血管(PTC)密度。结果模型组术后第7天部分肾小管萎缩,纤维化形成;术后第14天肾小管间质纤维化更加明显。假手术组eNOS表达较多,CTGF低表达。与假手术组相比,模型组第7、14天eNOS表达减少(P<0.05),CTGF表达增多(P<0.05),第3-14天PTC密度下降(P<0.05)。结论小鼠UUO肾组织eNOS表达下降参与了肾间质纤维化的进展。
Objective To investigate the changes of endothelial nitric oxide synthase (eNOS) in the kidney of unilateral ureteral obstruction (UUO) model and its relationship with renal interstitial fibrosis. Methods Forty-eight mice were randomly divided into UUO model group and sham operation group. Six mice were sacrificed on the 1st, 3rd, 7th and 14th day after operation respectively. The pathological changes of renal tissues were observed, and the growth of eNOS and connective tissue Factor (CTGF) expression, calculate the density of the tube capillary (PTC). Results On the 7th day after operation, part of the renal tubular atrophy and fibrosis were formed in the model group. Tubulointerstitial fibrosis was more obvious on the 14th day after the operation. The sham-operated group had more eNOS and lower CTGF expression. Compared with the sham-operated group, the eNOS expression decreased (P <0.05) and the expression of CTGF increased (P <0.05) on the 7th and 14th day in the model group, and decreased on the 3rd to 14th day (P <0.05). Conclusion The decreased expression of eNOS in the UUO kidney of mice is involved in the progression of renal interstitial fibrosis.