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1984年,我们对贵州地区马来丝虫及海南地区斑氏丝虫成虫形态进行了观察,现报告如下:材料和方法分别从沙鼠和恒河猴模型获取贵州地区人体马来丝虫((?)10,♂7)及海南地区斑氏丝虫成虫((?)4,♂25),置5%甘油酒精中固定,先进行光学显微镜观察,然后将成虫取出,以pH7.4缓冲生理盐水洗涤数次,3%戊二醛固定3~24小时,再以生理盐水冲洗数次至无戊二醛气味止;逐级乙醇脱水,每次3~5分钟,以乙酸乙酯替代15分钟,置于CO_2临界点干燥器内干燥,解剖镜下切取头、体、尾各部,在真空冷模仪内倾斜旋转喷金,送
In 1984, we observed the morphous of adult filariasis of Malayan filariasis in Guizhou Province and Hainan Island. The report is as follows: Materials and Methods We obtained the male adult filariasis from Guizhou Province ?) 10, ♂ 7) and adult filariasis (?) 4, ♂ 25) in Hainan, fixed in 5% glycerol alcohol, first observed by optical microscopy, and then remove the adult to pH7.4 buffer physiology Saline washed several times, 3% glutaraldehyde fixed 3 to 24 hours, and then rinse with saline several times to no glutaraldehyde only; gradual ethanol dehydration, each 3 to 5 minutes, with ethyl acetate instead of 15 minutes , Placed in the dryer at the critical point of CO_2 drying, dissecting microscope head, body, tail ministries, in the vacuum cold mold tilt tilt spray gold, send