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目的:建立稳定的动物腹膜转移癌模型,分析移植性腹膜转移癌的CT影像学特征,鉴定其生物学行为。方法:将新西兰大白兔36只分为开腹包埋、开腹穿刺及直接经皮穿刺种植3组(每组12只),每组又分为6只瘤块接种,6只悬液接种,接种VX2肿瘤。观察种植肿瘤生长状况,通过影像学及病理学检查分析局部区域及远处转移情况。结果:3种方法构建模型的成功率分别是100%(12/12)、91.7%(11/12)和58.3%(7/12),开腹包埋、开腹穿刺接种种植率差异无统计学意义(P>0.05),开腹与经皮穿刺比较,差异有统计学意义(P<0.05)。接种2周后,3组都能形成典型溃疡型胃癌并腹膜转移癌表现,CT上表现为占位性病变,呈稍高软组织密度结节,增强后肿瘤表现为明显或轻度强化,宿主衰竭,4周出现肺部转移。病理学检查符合典型VX2组织学特点。结论:开腹包埋与开腹穿刺接种制作VX2兔腹膜转移癌模型简单,实验周期短,接种率高,CT平扫和增强是检测肿瘤生长及血供的可靠方法,其病理表现类似人类腹膜转移癌,为腹膜癌治疗的实验研究提供可靠的大型动物模型。
OBJECTIVE: To establish a stable animal model of peritoneal metastasis and to analyze the CT imaging features of the transplanted peritoneal metastases and to identify their biological behavior. Methods: 36 New Zealand white rabbits were divided into three groups (12 in each group) under open laparotomy, open puncture and direct percutaneous puncture. Each group was divided into 6 tumor inoculation and 6 suspension inoculation. VX2 tumor inoculation. The growth of the tumor was observed and analyzed by imaging and pathology to analyze the local area and distant metastasis. Results: The successful rates of the three methods were 100% (12/12), 91.7% (11/12) and 58.3% (7/12), respectively. There was no statistical difference in the rate of laparotomy and laparotomy Significance (P> 0.05), laparotomy and percutaneous puncture, the difference was statistically significant (P <0.05). After 2 weeks of inoculation, all three groups were able to form typical ulcerated gastric cancer with peritoneal metastasis. CT showed a mass-nodular lesion with a slightly higher density of soft tissue nodules. The enhanced tumor showed obvious or slight enhancement and host failure , 4 weeks of lung metastasis. Pathological examination with typical VX2 histological features. Conclusion: The laparotomy and laparotomy inoculation of VX2 rabbit peritoneal metastasis model is simple, short experimental period, high vaccination rate, CT scan and enhancement is a reliable method for detecting tumor growth and blood supply, the pathological findings similar to the human peritoneum Metastatic cancer, for the experimental study of peritoneal cancer treatment to provide a reliable large animal model.