兔VX2腹膜转移癌模型的建立及CT表现

来源 :武汉大学学报(医学版) | 被引量 : 0次 | 上传用户:ig226
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
目的:建立稳定的动物腹膜转移癌模型,分析移植性腹膜转移癌的CT影像学特征,鉴定其生物学行为。方法:将新西兰大白兔36只分为开腹包埋、开腹穿刺及直接经皮穿刺种植3组(每组12只),每组又分为6只瘤块接种,6只悬液接种,接种VX2肿瘤。观察种植肿瘤生长状况,通过影像学及病理学检查分析局部区域及远处转移情况。结果:3种方法构建模型的成功率分别是100%(12/12)、91.7%(11/12)和58.3%(7/12),开腹包埋、开腹穿刺接种种植率差异无统计学意义(P>0.05),开腹与经皮穿刺比较,差异有统计学意义(P<0.05)。接种2周后,3组都能形成典型溃疡型胃癌并腹膜转移癌表现,CT上表现为占位性病变,呈稍高软组织密度结节,增强后肿瘤表现为明显或轻度强化,宿主衰竭,4周出现肺部转移。病理学检查符合典型VX2组织学特点。结论:开腹包埋与开腹穿刺接种制作VX2兔腹膜转移癌模型简单,实验周期短,接种率高,CT平扫和增强是检测肿瘤生长及血供的可靠方法,其病理表现类似人类腹膜转移癌,为腹膜癌治疗的实验研究提供可靠的大型动物模型。 OBJECTIVE: To establish a stable animal model of peritoneal metastasis and to analyze the CT imaging features of the transplanted peritoneal metastases and to identify their biological behavior. Methods: 36 New Zealand white rabbits were divided into three groups (12 in each group) under open laparotomy, open puncture and direct percutaneous puncture. Each group was divided into 6 tumor inoculation and 6 suspension inoculation. VX2 tumor inoculation. The growth of the tumor was observed and analyzed by imaging and pathology to analyze the local area and distant metastasis. Results: The successful rates of the three methods were 100% (12/12), 91.7% (11/12) and 58.3% (7/12), respectively. There was no statistical difference in the rate of laparotomy and laparotomy Significance (P> 0.05), laparotomy and percutaneous puncture, the difference was statistically significant (P <0.05). After 2 weeks of inoculation, all three groups were able to form typical ulcerated gastric cancer with peritoneal metastasis. CT showed a mass-nodular lesion with a slightly higher density of soft tissue nodules. The enhanced tumor showed obvious or slight enhancement and host failure , 4 weeks of lung metastasis. Pathological examination with typical VX2 histological features. Conclusion: The laparotomy and laparotomy inoculation of VX2 rabbit peritoneal metastasis model is simple, short experimental period, high vaccination rate, CT scan and enhancement is a reliable method for detecting tumor growth and blood supply, the pathological findings similar to the human peritoneum Metastatic cancer, for the experimental study of peritoneal cancer treatment to provide a reliable large animal model.
其他文献
请下载后查看,本文暂不支持在线获取查看简介。 Please download to view, this article does not support online access to view profile.
小麦全蚀病是我市小麦生产的重要威胁之一。据近几年观察,发病程度有逐年加重趋势。一九七五年在我市严重发生,成灾面积占小麦面积的45%。一九七六年,郊区各社队以阶级斗争为
随着桑蚕生产的发展,出现了一种新的桑树病害。据初步调查,我县三里桥、河东街、匡河、枫树铺、项家河等大队桑蚕场和大崎公社桑蚕场以及黄冈地区总路嘴蚕种场和浠水县前进
本文试图在借鉴外教上课经验的同时,结合自身的一些认识,对当前非英语专业本科生大学英语听力课提出一些改进意见。 This article attempts to draw lessons from the exper
本文分析了听力在大学英语教学中的重要性,大学英语听力教学的现状以及学生听力水平低的原因,并提出了提高大学英语听力课教学效果的途径。 This article analyzes the impo
请下载后查看,本文暂不支持在线获取查看简介。 Please download to view, this article does not support online access to view profile.
期刊
步入歌坛8年后,詹妮佛·洛佩兹终于在老公马克·安东尼的协助下发行了第一张母语专辑《Como Ama Una Mujer》(女人心)。这张唱片被拉丁音乐界列为年度强片,主打歌“Que Hicis
1976年从以来,我们对舞毒蛾(Ocneria dispar L.)和苹毒蛾(DasychiraPudibunda L.)的两种核型多角体病毒病进行了初步的观察。两虫均属鳞翅目,毒蛾科。食性很杂,是重要的园林
请下载后查看,本文暂不支持在线获取查看简介。 Please download to view, this article does not support online access to view profile.
期刊
探讨中药参阳方 (主药 :党参、黄芪、丹参、锁阳、女贞子等 )的急性与慢性毒性反应。进行常规的小鼠经口急性毒性试验 ,并通过建立移植瘤模型 ,观察该方对荷瘤裸鼠的长期慢性