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背景:白细胞介素1是强直性脊柱炎易感基因之一,其多态性与疾病易感性相关,不同人群的研究结果不尽相同。目的:分析中国湖北汉族人群中白细胞介素1受体拮抗剂基因多态性与强直性脊柱炎的相关性。方法:自140名强直性脊柱炎患者及130名健康对照者外周血中提取DNA,采用PCR方法检测白细胞介素1受体拮抗剂基因第二内含子VNTR复等位基因多态性。所有受试者均为3代或3代以上居住于湖北的汉族人。结果与结论:强直性脊柱炎患者中白细胞介素1受体拮抗剂基因*2等位基因频率显著高于健康对照者(P<0.05)。提示强直性脊柱炎与白细胞介素1受体拮抗剂基因*2等位基因相关联。
BACKGROUND: Interleukin-1 is one of the susceptibility genes of ankylosing spondylitis. Its polymorphism is related to disease susceptibility, and the results of different populations are not the same. OBJECTIVE: To analyze the association between interleukin-1 receptor antagonist gene polymorphism and ankylosing spondylitis in Han population in Hubei province of China. Methods: DNA was extracted from peripheral blood of 140 patients with ankylosing spondylitis and 130 healthy controls. The allele of the second intron of the interleukin 1 receptor antagonist gene (VNTR) was detected by PCR. All subjects were Han Chinese living in Hubei for 3 generations or more. RESULTS AND CONCLUSION: The frequency of allele * 2 of interleukin 1 receptor antagonist gene in patients with ankylosing spondylitis was significantly higher than that in healthy controls (P <0.05). It is suggested that ankylosing spondylitis is associated with the interleukin-1 receptor antagonist gene * 2 allele.