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目的:通过研究早、中、晚孕期胎盘因子(PF)对人外周血淋巴细胞(PBLs)中CD4,CCR5和CXCR4表达的作用,探讨PF在人免疫缺陷病毒-1(HIV-1)垂直传播中的作用及其机制。方法:制备早、中、晚孕期PF。分离人外周血单个核细胞,并分别与相对浓度为25%的早、中、晚孕期PF作用,培养24h后收集细胞,荧光抗体标记,流式细胞术检测外周血淋巴细胞(PBLs)中CD4,CCR5和CXCR4表达,以及CD4+T细胞中CCR5+细胞、CXCR4+细胞、CCR5+CXCR4+细胞所占的百分率。结果:各孕期PF均可显著降低PBLs中CCR5的表达,其中早孕期PF的作用明显强于中、晚孕期PF的作用;各孕期PF组CD4+T细胞中CCR5+细胞的百分率均显著低于对照组,早孕期PF组CD4+T细胞中CCR5+细胞的百分率明显低于中、晚孕期PF组;各孕期PF组CD4+T细胞中CCR5+CXCR4+细胞的百分率均显著低于对照组,早孕期PF组CD4+T细胞中CCR5+CXCR4+细胞的百分率显著低于晚孕期PF组。结论:各孕期PF均可显著降低PBLs中CCR5的表达,以及CD4+T细胞中CCR5+细胞和CCR5+CXCR4+细胞的百分率,早孕期PF作用最强,中、晚孕期PF效应相当,PF可能通过抑制R5病毒的入胞而具有抗R5病毒的作用,并可能在阻断HIV-1宫内感染中具有重要作用。
Objective: To investigate the effect of PF on the expression of CD4, CCR5 and CXCR4 in human peripheral blood lymphocytes (PBLs) in early, middle and late pregnancy to explore the role of PF in the vertical transmission of human immunodeficiency virus-1 (HIV-1) In the role and mechanism. Methods: Preparation of early, middle and late pregnancy PF. Peripheral blood mononuclear cells (PBMCs) were isolated from human peripheral blood mononuclear cells (PBMCs) and were treated with PF at a relative concentration of 25% for early, middle and late pregnancy respectively. After culturing for 24h, cells were collected and labeled with fluorescent antibody. Flow cytometry , CCR5 and CXCR4 expression as well as the percentage of CCR5 + cells, CXCR4 + cells and CCR5 + CXCR4 + cells in CD4 + T cells. Results: PF in each pregnancy significantly reduced the expression of CCR5 in PBLs. The effect of PF in early pregnancy was stronger than that in middle and late pregnancy. The percentage of CCR5 + cells in CD4 + T cells in PF group during pregnancy was significantly lower than that in control Group, the percentage of CCR5 + cells in CD4 + T cells in PF group in early pregnancy was significantly lower than that in PF group in middle and late pregnancy. The percentage of CCR5 + CXCR4 + cells in CD4 + T cells in PF group was significantly lower than that in control group in early pregnancy The percentage of CCR5 + CXCR4 + cells in the group of CD4 + T cells was significantly lower than that in the group of the second trimester PF. CONCLUSION: PF during pregnancy can significantly reduce the expression of CCR5 in PBLs and the percentage of CCR5 + cells and CCR5 + CXCR4 + cells in PBMCs. The effect of PF in early pregnancy is the strongest, and the effect of PF in middle and late pregnancy is comparable. R5 virus into the cell with anti-R5 virus role and may play an important role in blocking intrauterine infection of HIV-1.